Surface Characteristics of Different Dental Cements after Incubation with Adherent and Non-adherent Strains of Streptococcus Mutans
DOI:
https://doi.org/10.21270/archi.v14i1.6342Palavras-chave:
Cimentos de Resina, Dureza, Streptococcus mutansResumo
The aim of this study was to evaluate surface characteristics and Knoop hardness of two resin cements after incubation with adherent and non-adherent strains of Streptococcus mutans. The ATCC 25175 strain and a mutant isolated due to its low biofilm forming capacity and normal acidogenic capacity were used. Disks with 5 mm diameter and 2 mm thickness were fabricated with RelyX U200 and Allcem following the manufacturer’s instructions. Specimens were incubated in TSB medium with the adherent or non-adherent strains at 37ºC for 30 days, changing the culture medium every day, whereas control group specimens were incubated with sterile culture medium. Then, all specimens were subjected to microhardness Knoop. Representative specimens were examined using scanning electron microscopy (SEM). Tukey multiple comparisons test and unpaired t-test was used to compare two groups (α = 0.05). The resin cement RelyX U200 do not presented degradation or microhardness alteration under conditions tested. On the other hand, the resin cement Allcem presented more organic matrix degradation, exposing more filler particles after incubation with adherent strain than after incubation with the non-adherent strain. Microhardness of Allcem was smaller after incubation with ATCC strain than with non-adherent strain, and both presented smaller microhardness than the specimens of the control group. In conclusion, the resin cement RelyX U200 was not affected by the medium incubation with both adherent or non-adherent bacteria. The resin cement Allcem was more affected by the adherent bacteria strain then non-adherent regarding to surface topography.
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1. Bollen CM, Lambrechts P, Quirynen M. Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dent Mater 1997; 13:258-269.
2. Kawai K, Urano M, Ebisu S. Effect of surface roughness of porcelain on adhesion of bacteria and their synthesizing glucans. J Prosthet Dent 2000; 83:664-667.
3. Carlén A, Nikdel K, Wennerberg A, Holmberg K, Olsson J. Surface characteristics and in vitro biofilm formation on glass ionomer and composite resin. Biomaterials 2001; 22:481-487.
4. Pannu P, Gambhir R, Sujlana A. Correlation between the salivary Streptococcus mutans levels and dental caries experience in adult population of Chandigarh, India. Eur J Dent 2013; 7:191-195.
5. Chestnutt IG, MacFarlane TW, Stephen KW. An in vitro investigation of the cariogenic potential of oral Streptococci. Arch Oral Biol 1994; 39:589-593.
6. de Menezes FC, Junior GT, de Oliveira WJ, Paulino T de P, de Moura MB, da Silva IL, de Moura MB. Analysis of the properties of dental cements after exposure to incubation media containing Streptococcus mutans. J Contemp Dent Pract 2011; 12:385-391.
7. Krzyściak W, Jurczak A, Kościelniak D, Bystrowska B, Skalniak A. The virulence of Streptococcus mutans and the ability to form biofilms. Eur J Clin Microbiol Infect Dis 2014; 33:499-515.
8. Bowen WH, Koo H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res 2011; 45:69-86.
9. Aziz AM, El-Mowafy O, Tenenbaum HC, Lawrence HP. Clinical performance of CAD-CAM crowns provided by predoctoral students at the University of Toronto. J Prosthet Dent 2021; 7:S0022-3913(20)30716-2.
10. Gehrke P, Bleuel K, Fischer C, Sader R. Influence of margin location and luting material on the amount of undetected cement excess on CAD/CAM implant abutments and cement-retained zirconia crowns: an in-vitro study. BMC Oral Health 2019; 14;19(1):111.
11. Hao Y, Huang X, Zhou X, Li M, Ren B, Peng X, Cheng L. Influence of Dental Prosthesis and Restorative Materials Interface on Oral Biofilms. Int J Mol Sci 2018; 14;19(10):3157.
12. Astasov-Frauenhoffer M, Glauser S, Fischer J, Schmidli F, Waltimo T, Rohr N. Dent Mater 2018; 34:1702-1709.
13. Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol 1995; 22:1-
14. Souza JC, Mota RR, Sordi MB, Passoni BB, Benfatti CA, Magini RS. Biofilm formation on different materials used in oral rehabilitation. Braz Dent J 2016; 27:141-147.
15. Rohr N, Bertschinger N, Fischer J, Filippi A, Zitzmann NU. Influence of material and surface roughness of resin composite cements on fibroblast behavior. Oper Dent 2020; 45:528-536.
16. Glauser S, Astasov-Frauenhoffer M, Müller JA, Fischer J, Waltimo T, Rohr N. Bacterial colonization of resin composite cements: influence of material composition and surface roughness. Eur J Oral Sci 2017; 125:294-302.
17. Aguiar TR, Di Francescantonio M, Bedran-Russo AK, Giannini M. Inorganic composition and filler particles morphology of conventional and self-adhesive resin cements by SEM/EDX. Microsc Res Tech 2012; 75:1348-1352.